BJBS Article :: Molecular characterisation of verocytoxigenic Escherichia coli O157:H7 by random amplification of polymorphic DNA (RAPD) typing

Molecular characterisation of verocytoxigenic Escherichia coli O157:H7 by random amplification of polymorphic DNA (RAPD) typing

IN BRIEF: Br J Biomed Sci 2008; 65(3); 161-163

JE Moore^*, M Watabe, BC Millar^*, MAS McMahon^†, DA McDowell^†, PJ Rooney, A Loughrey^*, CE Goldsmith^*

^*Northern Ireland Public Health Laboratory, Belfast City Hospital, Northern Ireland, UK
^†School of Health Sciences, University of Ulster, Jordanstown, Newtownabbey, Co Antrim, Northern Ireland, UK

KEY WORDS:

For the past two decades, polymerase chain reaction (PCR)- based genotyping methods have played an important role in bacterial typing schemes. One such PCR-based method, random amplification of polymorphic DNA (RAPD)-PCR, also known as arbitrarily primed-PCR (AP-PCR), has proved useful on account of its simplicity and utility for analysis of large-throughput samples.1 This technique utilises a variable short-length arbitrary primer and does not require previous knowledge of the target DNA sequence data. The primer is amplified arbitrarily at low stringency, where the oligonucleotide binds at complementary and partially mismatched sites and generates bands that differ in length and nucleotide composition.[Contd]

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